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Yes. It's less common to use 'guys' to refer to females unless you are referring to a group of males and females together. In that case 'guys' is an informal substitute for 'people'. If you're talking to people directly 'guys' is acceptable, e.g. 'Hi guys!' even if some (or all) are female. In this context it means 'friends' or 'acquaintances'. You wouldn't refer to a group of females indirectly as 'guys' e.g. 'Look at those guys over there'. You'd say 'ladies' or 'girls' or 'women' (assuming you need to mention their gender). As a rule of thumb I'd say people use 'guys' when addressing a group containing males (with or without females) OR when directly addressing people informally, such as friends. In a more formal or less familiar context you'd refer to a group of females as 'ladies' or 'girls'. The usage of this word is far more nuanced than I ever thought...I don't think it used to be used when addressing females at all, but it certainly is more gender neutral now.
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